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Nat Rev Cancer. 2015 Jul;15(7):387-95. doi: 10.1038/nrc3950. Epub 2015 Jun 4.

Applications of the CRISPR-Cas9 system in cancer biology.

Author information

1
David H. Koch Institute for Integrative Cancer Research and the Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA.
2
1] David H. Koch Institute for Integrative Cancer Research and the Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA. [2] Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Abstract

The prokaryotic type II CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR-associated 9) system is rapidly revolutionizing the field of genetic engineering, allowing researchers to alter the genomes of a large range of organisms with relative ease. Experimental approaches based on this versatile technology have the potential to transform the field of cancer genetics. Here, we review current approaches for functional studies of cancer genes that are based on CRISPR-Cas, with emphasis on their applicability for the development of next-generation models of human cancer.

PMID:
26040603
PMCID:
PMC4530801
DOI:
10.1038/nrc3950
[Indexed for MEDLINE]
Free PMC Article

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