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Adv Healthc Mater. 2015 Aug 5;4(11):1628-33. doi: 10.1002/adhm.201500021. Epub 2015 Jun 3.

Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture.

Author information

1
School of Engineering and Applied Sciences, Harvard University, 02138, Cambridge, MA, USA.
2
Faculty of Chemistry, University of Belgrade, Studentski trg 12, 11000, Belgrade, Serbia.
3
Molecular Biotechnology, Faculty of Biology, RWTH Aachen University, 52062, Aachen, Germany.
4
Wyss Institute for Biologically Inspired Engineering, 02138, Cambridge, MA, USA.
5
Department of Physics, Harvard University, 02138, Cambridge, MA, USA.

Abstract

Monodisperse alginate microgels (10-50 μm) are created via droplet-based microfluidics by a novel crosslinking procedure. Ionic crosslinking of alginate is induced by release of chelated calcium ions. The process separates droplet formation and gelation reaction enabling excellent control over size and homogeneity under mild reaction conditions. Living mesenchymal stem cells are encapsulated and cultured in the generated 3D microenvironments.

KEYWORDS:

alginate; cells; encapsulation; microfluidics; microgels

PMID:
26039892
PMCID:
PMC4529809
DOI:
10.1002/adhm.201500021
[Indexed for MEDLINE]
Free PMC Article

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