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Biosci Biotechnol Biochem. 2015;79(11):1852-9. doi: 10.1080/09168451.2015.1050988. Epub 2015 Jun 3.

Monodisperse aqueous microspheres encapsulating high concentration of l-ascorbic acid: insights of preparation and stability evaluation from straight-through microchannel emulsification.

Author information

a Food Engineering Division , National Food Research Institute, NARO , Tsukuba , Japan.
b Graduate School of Agricultural and Life Sciences , The University of Tokyo , Bunkyo-ku , Japan.
c Faculty of Life and Environmental Sciences , University of Tsukuba , Tsukuba , Japan.


Stabilization of l-ascorbic acid (l-AA) is a challenging task for food and pharmaceutical industries. The study was conducted to prepare monodisperse aqueous microspheres containing enhanced concentrations of l-AA by using microchannel emulsification (MCE). The asymmetric straight-through microchannel (MC) array used here constitutes 11 × 104 μm microslots connected to a 10 μm circular microholes. 5-30% (w/w) l-AA was added to a Milli-Q water solution containing 2% (w/w) sodium alginate and 1% (w/w) magnesium sulfate, while the continuous phase constitutes 5% (w/w) tetraglycerol condensed ricinoleate in water-saturated decane. Monodisperse aqueous microspheres with average diameters (dav) of 18.7-20.7 μm and coefficients of variation (CVs) below 6% were successfully prepared via MCE regardless of the l-AA concentrations applied. The collected microspheres were physically stable in terms of their dav and CV for >10 days of storage at 40°C. The aqueous microspheres exhibited l-AA encapsulation efficiency exceeding 70% during the storage.


high concentration; l-ascorbic acid; monodisperse microspheres; sodium alginate; stability

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