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J Exp Bot. 2015 Sep;66(19):5739-52. doi: 10.1093/jxb/erv274. Epub 2015 Jun 2.

Comprehensive transcript profiling of two grapevine rootstock genotypes contrasting in drought susceptibility links the phenylpropanoid pathway to enhanced tolerance.

Author information

1
Department of Agronomy, Food, Natural resources, Animals and Environment (DAFNAE), University of Padova Agripolis, 35020 Legnaro, Italy Centro Interdipartimentale per la Ricerca in Viticoltura ed Enologia (CIRVE), Via XXVIII Aprile, 14-31015 Conegliano (TV), Italy.
2
Genomics and Biotechnology of Fruit (GBF) Laboratory, Institut National Polytechnique de Toulouse, Avenue de l'Agrobiopole, F-31326 Castanet-Tolosan Cedex (Toulouse), France.
3
CRIBI, University of Padova, viale G. Colombo 3, 35121 Padova, Italy.
4
Department of Agricultural and Environmental Sciences-Production, Landscape, Agroenergy (DiSAA), University of Milano, Milano 20133, Italy.
5
Department of Agronomy, Food, Natural resources, Animals and Environment (DAFNAE), University of Padova Agripolis, 35020 Legnaro, Italy Centro Interdipartimentale per la Ricerca in Viticoltura ed Enologia (CIRVE), Via XXVIII Aprile, 14-31015 Conegliano (TV), Italy claudio.bonghi@unipd.it.

Abstract

In light of ongoing climate changes in wine-growing regions, the selection of drought-tolerant rootstocks is becoming a crucial factor for developing a sustainable viticulture. In this study, M4, a new rootstock genotype that shows tolerance to drought, was compared from a genomic and transcriptomic point of view with the less drought-tolerant genotype 101.14. The root and leaf transcriptome of both 101.14 and the M4 rootstock genotype was analysed, following exposure to progressive drought conditions. Multifactorial analyses indicated that stress treatment represents the main factor driving differential gene expression in roots, whereas in leaves the genotype is the prominent factor. Upon stress, M4 roots and leaves showed a higher induction of resveratrol and flavonoid biosynthetic genes, respectively. The higher expression of VvSTS genes in M4, confirmed by the accumulation of higher levels of resveratrol in M4 roots compared with 101.14, was coupled to an up-regulation of several VvWRKY transcription factors. Interestingly, VvSTS promoter analyses performed on both the resequenced genomes highlighted a significantly higher number of W-BOX elements in the tolerant genotype. It is proposed that the elevated synthesis of resveratrol in M4 roots upon water stress could enhance the plant's ability to cope with the oxidative stress usually associated with water deficit.

KEYWORDS:

Flavonoids; Vitis; genome re-sequencing; mRNA-Seq; stilbenes; water stress.

PMID:
26038306
PMCID:
PMC4566973
DOI:
10.1093/jxb/erv274
[Indexed for MEDLINE]
Free PMC Article

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