Format

Send to

Choose Destination
Plant Cell Environ. 2015 Dec;38(12):2721-34. doi: 10.1111/pce.12583. Epub 2015 Jul 3.

ETHYLENE RESPONSE FACTOR 96 positively regulates Arabidopsis resistance to necrotrophic pathogens by direct binding to GCC elements of jasmonate - and ethylene-responsive defence genes.

Author information

1
Department of Life Science and Institute of Plant Biology, National Taiwan University, Taipei, Taiwan.
2
Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan.
3
Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
4
Department of Life Sciences, National Central University, Taoyuan, Taiwan.
5
Department of Life Science and Biotechnology Center, National Chung Hsing University, Taichung, Taiwan.

Abstract

The ERF (ethylene responsive factor) family is composed of transcription factors (TFs) that are critical for appropriate Arabidopsis thaliana responses to biotic and abiotic stresses. Here we identified and characterized a member of the ERF TF group IX, namely ERF96, that when overexpressed enhances Arabidopsis resistance to necrotrophic pathogens such as the fungus Botrytis cinerea and the bacterium Pectobacterium carotovorum. ERF96 is jasmonate (JA) and ethylene (ET) responsive and ERF96 transcripts accumulation was abolished in JA-insensitive coi1-16 and in ET-insensitive ein2-1 mutants. Protoplast transactivation and electrophoresis mobility shift analyses revealed that ERF96 is an activator of transcription that binds to GCC elements. In addition, ERF96 mainly localized to the nucleus. Microarray analysis coupled to chromatin immunoprecipitation-PCR of Arabidopsis overexpressing ERF96 revealed that ERF96 enhances the expression of the JA/ET defence genes PDF1.2a, PR-3 and PR-4 as well as the TF ORA59 by direct binding to GCC elements present in their promoters. While ERF96-RNAi plants demonstrated wild-type resistance to necrotrophic pathogens, basal PDF1.2 expression levels were reduced in ERF96-silenced plants. This work revealed ERF96 as a key player of the ERF network that positively regulates the Arabidopsis resistance response to necrotrophic pathogens.

KEYWORDS:

Arabidopsis thaliana; Botrytis cinerea; Pectobacterium carotovorum; ethylene; ethylene responsive factor; innate immunity; necrotroph

PMID:
26038230
DOI:
10.1111/pce.12583
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center