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Biopreserv Biobank. 2015 Jun;13(3):195-9. doi: 10.1089/bio.2014.0073.

Establishment and cryopreservation of a giant panda skeletal muscle-derived cell line.

Author information

1
1College of Life Sciences, Zhejiang University, Hangzhou, China.
2
2Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Ya'an, Sichuan Province, China.
3
5Key Laboratory for Natural Resources Protection and Sustainable Utilization of Sichuan Province, Chengdu, China.
4
3China Conservation and Research Center for the Giant Panda, Wolong, China.
5
4Sichuan Province Natural Resources Science Academy, Chengdu, China.

Abstract

The giant panda Ailuropoda melanoleuca is an endangered species and is a symbol for wildlife conservation. Although efforts have been made to protect this rare and endangered species through breeding and conservative biology, the long-term preservation of giant panda genome resources (gametes, tissues, organs, genomic libraries, etc.) is still a practical option. In this study, the giant panda skeletal muscle-derived cell line was successfully established via primary explants culture and cryopreservation techniques. The population doubling time of giant panda skeletal cells was approximately 33.8 h, and this population maintained a high cell viability before and after cryopreservation (95.6% and 90.7%, respectively). The two skeletal muscle-specific genes SMYD1 and MYF6 were expressed and detected by RT-PCR in the giant panda skeletal muscle-derived cell line. Karyotyping analysis revealed that the frequencies of giant panda skeletal muscle cells showing a chromosome number of 2n=42 ranged from 90.6∼94.2%. Thus, the giant panda skeletal muscle-derived cell line provides a vital resource and material platform for further studies and is likely to be useful for the protection of this rare and endangered species.

PMID:
26035009
DOI:
10.1089/bio.2014.0073
[Indexed for MEDLINE]

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