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Biopreserv Biobank. 2015 Jun;13(3):195-9. doi: 10.1089/bio.2014.0073.

Establishment and cryopreservation of a giant panda skeletal muscle-derived cell line.

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1College of Life Sciences, Zhejiang University, Hangzhou, China.
2Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Ya'an, Sichuan Province, China.
5Key Laboratory for Natural Resources Protection and Sustainable Utilization of Sichuan Province, Chengdu, China.
3China Conservation and Research Center for the Giant Panda, Wolong, China.
4Sichuan Province Natural Resources Science Academy, Chengdu, China.


The giant panda Ailuropoda melanoleuca is an endangered species and is a symbol for wildlife conservation. Although efforts have been made to protect this rare and endangered species through breeding and conservative biology, the long-term preservation of giant panda genome resources (gametes, tissues, organs, genomic libraries, etc.) is still a practical option. In this study, the giant panda skeletal muscle-derived cell line was successfully established via primary explants culture and cryopreservation techniques. The population doubling time of giant panda skeletal cells was approximately 33.8 h, and this population maintained a high cell viability before and after cryopreservation (95.6% and 90.7%, respectively). The two skeletal muscle-specific genes SMYD1 and MYF6 were expressed and detected by RT-PCR in the giant panda skeletal muscle-derived cell line. Karyotyping analysis revealed that the frequencies of giant panda skeletal muscle cells showing a chromosome number of 2n=42 ranged from 90.6∼94.2%. Thus, the giant panda skeletal muscle-derived cell line provides a vital resource and material platform for further studies and is likely to be useful for the protection of this rare and endangered species.

[Indexed for MEDLINE]

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