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Cytometry A. 2015 Oct;87(10):967-75. doi: 10.1002/cyto.a.22689. Epub 2015 Jun 1.

Design and validation of conditional ligands for HLA-B*08:01, HLA-B*15:01, HLA-B*35:01, and HLA-B*44:05.

Author information

1
Department of Hematology, Center for Cancer Immune Therapy, Herlev University Hospital, Copenhagen, Denmark.
2
Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
3
Section for Immunology and Vaccinology, National Veterinary Institute, Technical University of Denmark, Copenhagen, Denmark.
4
Department of Microbiology, National University of Singapore, Singapore.
5
Department of Biological Sciences and the Immunology Program, National University of Singapore, Singapore.
6
Department of Oncology, Herlev University Hospital, Copenhagen, Denmark.
7
Department of Hematology, Leiden University Medical Centre, Leiden, The Netherlands.

Abstract

We designed conditional ligands restricted to HLA-B*08:01, -B*35:01, and -B*44:05 and proved the use of a conditional ligand previously designed for HLA-B*15:02 together with HLA-B*15:01. Furthermore, we compared the detection capabilities of specific HLA-B*15:01-restricted T cells using the HLA-B*15:01 and HLA-B*15:02 major histocompatibility complex (MHC) multimers and found remarkable differences in the staining patterns detected by flow cytometry. These new conditional ligands greatly add to the application of MHC-based technologies in the analyses of T-cell recognition as they represent frequently expressed HLA-B molecules. This expansion of conditional ligands is important to allow T-cell detection over a wide range of HLA restrictions, and provide comprehensive understanding of the T-cell recognition in a given context.

KEYWORDS:

CD8 T cells; HLA-B molecules; MHC multimer; conditional ligands; flow cytometry

PMID:
26033882
DOI:
10.1002/cyto.a.22689
[Indexed for MEDLINE]
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