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Opt Lett. 2015 Jun 1;40(11):2653-6. doi: 10.1364/OL.40.002653.

Dual-color three-dimensional STED microscopy with a single high-repetition-rate laser.

Abstract

We describe a dual-color three-dimensional stimulated emission depletion (3D-STED) microscopy employing a single laser source with a repetition rate of 80 MHz. Multiple excitation pulses synchronized with a STED pulse were generated by a photonic crystal fiber, and the desired wavelengths were selected by an acousto-optic tunable filter with high spectral purity. Selective excitation at different wavelengths permits simultaneous imaging of two fluorescent markers at a nanoscale resolution in three dimensions.

PMID:
26030581
PMCID:
PMC4849877
DOI:
10.1364/OL.40.002653
[Indexed for MEDLINE]
Free PMC Article

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