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Nat Methods. 2015 Jul;12(7):631-3. doi: 10.1038/nmeth.3432. Epub 2015 Jun 1.

Plasmodium falciparum genetic crosses in a humanized mouse model.

Author information

1
Center for Infectious Disease Research (formerly Seattle Biomedical Research Institute), Seattle, Washington, USA.
2
Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana, USA.
3
Texas Biomedical Research Institute, San Antonio, Texas, USA.
4
Shoklo Malaria Research Unit, Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Mae Sot, Thailand.
5
1] Center for Infectious Disease Research (formerly Seattle Biomedical Research Institute), Seattle, Washington, USA. [2] Department of Global Health, University of Washington, Seattle, Washington, USA.

Abstract

Genetic crosses of phenotypically distinct strains of the human malaria parasite Plasmodium falciparum are a powerful tool for identifying genes controlling drug resistance and other key phenotypes. Previous studies relied on the isolation of recombinant parasites from splenectomized chimpanzees, a research avenue that is no longer available. Here we demonstrate that human-liver chimeric mice support recovery of recombinant progeny for the identification of genetic determinants of parasite traits and adaptations.

PMID:
26030447
PMCID:
PMC4547688
DOI:
10.1038/nmeth.3432
[Indexed for MEDLINE]
Free PMC Article

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