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Nat Methods. 2015 Jul;12(7):637-40. doi: 10.1038/nmeth.3411. Epub 2015 Jun 1.

Functional differentiation of human pluripotent stem cells on a chip.

Author information

1
1] Department of Industrial Engineering, University of Padova, Padova, Italy. [2] Venetian Institute of Molecular Medicine, Padova, Italy.
2
Department of Molecular Medicine, University of Padova, Padova, Italy.
3
Department of Surgery, Oncology and Gastroenterology, University of Padova, Padova, Italy.

Abstract

Microengineering human "organs-on-chips" remains an open challenge. Here, we describe a robust microfluidics-based approach for the differentiation of human pluripotent stem cells directly on a chip. Extrinsic signal modulation, achieved through optimal frequency of medium delivery, can be used as a parameter for improved germ layer specification and cell differentiation. Human cardiomyocytes and hepatocytes derived on chips showed functional phenotypes and responses to temporally defined drug treatments.

PMID:
26030445
DOI:
10.1038/nmeth.3411
[Indexed for MEDLINE]

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