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Mol Cell. 2015 Jun 4;58(5):722-8. doi: 10.1016/j.molcel.2015.05.023. Epub 2015 May 28.

A Conserved Structural Chassis for Mounting Versatile CRISPR RNA-Guided Immune Responses.

Author information

1
Department of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA.
2
Department of Microbiology and Immunology, Montana State University, Bozeman, MT 59717, USA. Electronic address: bwiedenheft@gmail.com.

Abstract

Bacteria and archaea rely on CRISPR (clustered regularly interspaced short palindromic repeats) RNA-guided adaptive immune systems for targeted elimination of foreign nucleic acids. These immune systems have been divided into three main types, and the first atomic-resolution structure of a type III RNA-guided immune complex provides new insights into the mechanisms of nucleic acid degradation. Here we compare the crystal structure of a type III complex to recently determined structures of DNA-targeting type I CRISPR complexes. Structural comparisons support previous assertions that type I and type III systems share a common ancestor and reveal how a conserved structural chassis is used to support RNA-, DNA-, or both RNA- and DNA-targeting mechanisms.

PMID:
26028539
PMCID:
PMC4465340
DOI:
10.1016/j.molcel.2015.05.023
[Indexed for MEDLINE]
Free PMC Article

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