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Cell Rep. 2015 Jun 16;11(10):1511-8. doi: 10.1016/j.celrep.2015.05.008. Epub 2015 May 28.

Bidirectional Interplay between Vimentin Intermediate Filaments and Contractile Actin Stress Fibers.

Author information

1
Institute of Biotechnology, P.O. Box 56, 00014 University of Helsinki, Helsinki, Finland.
2
Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, 20521 Turku, Finland; Department of Biosciences, Åbo Akademi University, 20520 Turku, Finland.
3
Institute of Biotechnology, P.O. Box 56, 00014 University of Helsinki, Helsinki, Finland. Electronic address: pekka.lappalainen@helsinki.fi.

Abstract

The actin cytoskeleton and cytoplasmic intermediate filaments contribute to cell migration and morphogenesis, but the interplay between these two central cytoskeletal elements has remained elusive. Here, we find that specific actin stress fiber structures, transverse arcs, interact with vimentin intermediate filaments and promote their retrograde flow. Consequently, myosin-II-containing arcs are important for perinuclear localization of the vimentin network in cells. The vimentin network reciprocally restricts retrograde movement of arcs and hence controls the width of flat lamellum at the leading edge of the cell. Depletion of plectin recapitulates the vimentin organization phenotype of arc-deficient cells without affecting the integrity of vimentin filaments or stress fibers, demonstrating that this cytoskeletal cross-linker is required for productive interactions between vimentin and arcs. Collectively, our results reveal that plectin-mediated interplay between contractile actomyosin arcs and vimentin intermediate filaments controls the localization and dynamics of these two cytoskeletal systems and is consequently important for cell morphogenesis.

PMID:
26027931
DOI:
10.1016/j.celrep.2015.05.008
[Indexed for MEDLINE]
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