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Toxicon. 2015 Aug;102:62-8. doi: 10.1016/j.toxicon.2015.05.015. Epub 2015 May 27.

Prevalence of ciguatoxins in lionfish (Pterois spp.) from Guadeloupe, Saint Martin, and Saint Barthélmy Islands (Caribbean).

Author information

1
Institut de la Recerca i Tecnologia Agroalimentàries (IRTA), Ctra. Poble Nou Km 5,5, Sant Carles de la Ràpita, E-43540, Spain.
2
Comité Régional des Pêches Maritimes et des Élevages Marins (CRPMEM), Rue Schoelcher 2 bis, Pointe a Pitre, 97110, Guadeloupe.
3
Association Réunionnaise pour le Développement de 'Aquaculture (ARDA) et ARVAM, C/o CIROY, 2, Rue Maxime Rivière, 97490, Sainte Clotilde, Reunion.
4
U.S. Food and Drug Administration (FDA), Division of Seafood Science and Technology, Gulf Coast Seafood Laboratory, 1 Iberville Drive, Dauphin Island, AL, 36528, USA.
5
Institut de la Recerca i Tecnologia Agroalimentàries (IRTA), Ctra. Poble Nou Km 5,5, Sant Carles de la Ràpita, E-43540, Spain. Electronic address: jorge.diogene@irta.cat.

Abstract

Lionfish (Pterois spp.) are invasive species that have recently spread throughout the Caribbean. Lionfish are available for purchase in local markets for human consumption in several islands of the region. We examined the prevalence of ciguatoxins (CTXs) in lionfish from the French Antilles, a ciguatera-endemic region. The neuroblastoma-2a (N2a) cell assay was used to assess composite cytotoxicity in 120 fish samples collected from the surrounding waters of Guadeloupe (n = 60), Saint Barthélemy Islands (n = 55) and Saint Martin (n = 5). Twenty-seven of these samples exhibited CTX-like activity by the N2a assay. Ciguatoxin (CTX) was confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in multiple samples that presented highest composite toxicity levels by N2a. Those fish found to contain CTXs were all from Saint Barthélemy. Lionfish from Guadeloupe and Saint Martin did not exhibit toxin activity, although the sample size from Saint Martin was insufficient to draw any conclusions as to the incidence of CTXs. In this study, we provide information about the potential hazard of ciguatera associated with the consumption of lionfish from known endemic areas. We also demonstrate the utility of the cell-based assay combined with LC-MS/MS to assess activity and to provide structural confirmation of CTXs respectively.

KEYWORDS:

Caribbean; Ciguatera; Ciguatoxin; Guadeloupe; Lionfish; Pterois spp.

PMID:
26026621
DOI:
10.1016/j.toxicon.2015.05.015
[Indexed for MEDLINE]

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