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Sci Rep. 2015 May 29;5:10658. doi: 10.1038/srep10658.

RiboTALE: A modular, inducible system for accurate gene expression control.

Author information

1
UC Davis Genome Center, University of California-Davis, Davis, CA, USA.
2
UC Davis Undergraduate Program, University of California-Davis, Davis, CA, USA.
3
1] UC Davis Genome Center, University of California-Davis, Davis, CA, USA [2] Department of Biomedical Engineering, University of California-Davis, Davis, CA, USA.
4
1] UC Davis Genome Center, University of California-Davis, Davis, CA, USA [2] Department of Biochemistry &Molecular Medicine, University of California-Davis, Davis, CA, USA [3] Department of Chemistry, University of California-Davis, Davis, CA, USA.
5
1] UC Davis Genome Center, University of California-Davis, Davis, CA, USA [2] Department of Computer Science, University of California-Davis, Davis, CA, USA.

Abstract

A limiting factor in synthetic gene circuit design is the number of independent control elements that can be combined together in a single system. Here, we present RiboTALEs, a new class of inducible repressors that combine the specificity of TALEs with the ability of riboswitches to recognize exogenous signals and differentially control protein abundance. We demonstrate the capacity of RiboTALEs, constructed through different combinations of TALE proteins and riboswitches, to rapidly and reproducibly control the expression of downstream targets with a dynamic range of 243.7 ± 17.6-fold, which is adequate for many biotechnological applications.

PMID:
26023068
PMCID:
PMC4650599
DOI:
10.1038/srep10658
[Indexed for MEDLINE]
Free PMC Article

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