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Plasmid. 2015 Sep;81:21-6. doi: 10.1016/j.plasmid.2015.03.001. Epub 2015 May 27.

A series of medium and high copy number arabinose-inducible Escherichia coli expression vectors compatible with pBR322 and pACYC184.

Author information

1
Department of Microbiology, University of Illinois, B103 Chemical and Life Sciences Laboratory, 601S. Goodwin Ave., Urbana, IL 61801, USA.
2
Department of Microbiology, University of Illinois, B103 Chemical and Life Sciences Laboratory, 601S. Goodwin Ave., Urbana, IL 61801, USA; Department of Biochemistry, University of Illinois, 419 Roger Adams Laboratory, 600 South Mathews Ave., Urbana, IL 61801, USA. Electronic address: j-cronan@life.uiuc.edu.

Abstract

The original pBAD24 plasmid and the derived lower copy number (the pBAD322 series) expression vectors have been widely used in Escherichia coli, Salmonella enterica, and related bacteria. However, a flexible pBAD expression system has been available only in pMB1 (ColE1) vectors. We report a series of pBAD vectors that replicate using the origin of plasmid RSF1030 that are compatible with pMB1 (ColE1) and p15A (pACYC) vectors. Both high (≥pBAD24) and medium (~pBAD322) copy number plasmids encoding resistance to ampicillin, chloramphenicol, kanamycin, tetracycline, spectinomycin/streptomycin, gentamycin, or trimethoprim are available.

KEYWORDS:

Copy number; Expression levels; Plasmid Compatibility; RSF1030; pBAD24; pBR322

PMID:
26021570
PMCID:
PMC4600428
DOI:
10.1016/j.plasmid.2015.03.001
[Indexed for MEDLINE]
Free PMC Article

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