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Biotechnol Biotechnol Equip. 2014 Nov 2;28(6):1024-1033. Epub 2014 Nov 28.

Biotransformation of steroidal saponins in sisal (Agave sisalana Perrine) to tigogenin by a newly isolated strain from a karst area of Guilin, China.

Author information

1
College of Chemistry and Bioengineering, Guilin University of Technology , Guilin , China ; College of Life Science, Northeast Agriculture University , Harbin , China.
2
College of Chemistry and Bioengineering, Guilin University of Technology , Guilin , China.
3
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences , Haikou , China.
4
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences , Beijing , China.

Abstract

A rod-shaped bacterium was isolated from the soil in a karst area of Guilin, China and its biotransformation of steroidal saponins in sisal (Agave sisalana Perrine) to tigogenin was presented for the first time. A total of 22 strains for the degradation of steroidal saponins in sisal were isolated from 48 soil samples, and the isolated rod-shaped, bacterial strain ZG-21 was used for the production of tigogenin due to its highest degradation efficiency of steroidal saponins in sisal. The parameters affecting biotransformation by strain ZG-21 were optimized. Under the optimized conditions of temperature (30 °C), pH (6), time (5 days) and substrate concentration (5 mg/mL), a maximum tigogenin yield of 26.7 mg/g was achieved. Compared with the conventional method of acid hydrolysis, the biotransformation method provided a clean and eco-friendly alternative for the production of tigogenin.

KEYWORDS:

acid hydrolysis; biotransformation; sisal; steroidal saponins; tigogenin

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