MiR-451 inhibits proliferation of esophageal carcinoma cell line EC9706 by targeting CDKN2D and MAP3K1

World J Gastroenterol. 2015 May 21;21(19):5867-76. doi: 10.3748/wjg.v21.i19.5867.

Abstract

Aim: To investigate the underlying molecular mechanisms of miR-451 to inhibit proliferation of esophageal carcinoma cell line EC9706.

Methods: Assays for cell growth, apoptosis and invasion were used to evaluate the effects of miR-451 expression on EC cells. Luciferase reporter and Western blot assays were used to test whether cyclin-dependent kinase inhibitor 2D (CDKN2D) and MAP3K1 act as major targets of miR-451.

Results: The results showed that CDKN2D and MAP3K1 are direct targets of miR-451. CDKN2D and MAP3K1 overexpression reversed the effect of miR-451. MiR-451 inhibited the proliferation of EC9706 by targeting CDKN2D and MAP3K1.

Conclusion: These findings suggest that miR-451 might be a novel prognostic biomarker and a potential target for the treatment of esophageal squamous cell carcinoma in the future.

Keywords: Cyclin-dependent kinase inhibitor 2D; Esophageal squamous cell carcinoma; MAP3K1; MiR-451; Proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinoma / enzymology*
  • Carcinoma / genetics
  • Carcinoma / pathology
  • Carcinoma, Squamous Cell / enzymology*
  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / pathology
  • Cell Line, Tumor
  • Cell Proliferation*
  • Cyclin-Dependent Kinase Inhibitor p19 / genetics
  • Cyclin-Dependent Kinase Inhibitor p19 / metabolism*
  • Esophageal Neoplasms / enzymology*
  • Esophageal Neoplasms / genetics
  • Esophageal Neoplasms / pathology
  • Esophageal Squamous Cell Carcinoma
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Neoplastic
  • Humans
  • MAP Kinase Kinase Kinase 1 / genetics
  • MAP Kinase Kinase Kinase 1 / metabolism*
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Signal Transduction
  • Transfection

Substances

  • CDKN2D protein, human
  • Cyclin-Dependent Kinase Inhibitor p19
  • MIRN451 microRNA, human
  • MicroRNAs
  • MAP Kinase Kinase Kinase 1
  • MAP3K1 protein, human