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Biomed Chromatogr. 2015 Dec;29(12):1921-31. doi: 10.1002/bmc.3517. Epub 2015 Jun 21.

A sensitive and efficient method for trace analysis of some phenolic compounds using simultaneous derivatization and air-assisted liquid-liquid microextraction from human urine and plasma samples followed by gas chromatography-nitrogen phosphorous detection.

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Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Food and Drug Laboratories, Tabriz University of Medical Sciences, Tabriz, Iran.


In present study, a simultaneous derivatization and air-assisted liquid-liquid microextraction method combined with gas chromatography-nitrogen phosphorous detection has been developed for the determination of some phenolic compounds in biological samples. The analytes are derivatized and extracted simultaneously by a fast reaction with 1-flouro-2,4-dinitrobenzene under mild conditions. Under optimal conditions low limits of detection in the range of 0.05-0.34 ng mL(-1) are achievable. The obtained extraction recoveries are between 84 and 97% and the relative standard deviations are less than 7.2% for intraday (n = 6) and interday (n = 4) precisions. The proposed method was demonstrated to be a simple and efficient method for the analysis of phenols in biological samples.


1-flouro-2,4-dinitrobenzene; air-assisted liquid-liquid microextraction; gas chromatography; nitrogen-phosphorous detector; phenolic compounds

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