Format

Send to

Choose Destination
Neuroscience. 2015 Aug 6;300:585-92. doi: 10.1016/j.neuroscience.2015.05.041. Epub 2015 May 23.

Voxel-based morphometry of the marmoset brain: In vivo detection of volume loss in the substantia nigra of the MPTP-treated Parkinson's disease model.

Author information

1
Department of Physiology, Keio University School of Medicine, Tokyo, Japan; Central Institute for Experimental Animals, Kawasaki, Japan.
2
Central Institute for Experimental Animals, Kawasaki, Japan.
3
Department of Physiology, Keio University School of Medicine, Tokyo, Japan.
4
Faculty of Radiological Technology, Fujita Health University School of Health Sciences, Toyoake, Japan.
5
Department of Diagnostic Radiology, Keio University School of Medicine, Tokyo, Japan.
6
Division of Regenerative Medicine, Jikei University School of Medicine, Tokyo, Japan.
7
Department of Physiology, Keio University School of Medicine, Tokyo, Japan; Laboratory for Marmoset Neural Architecture, Brain Science Institute RIKEN, Wako, Japan. Electronic address: hidokano@a2.keio.jp.

Abstract

Movement dysfunction in Parkinson's disease (PD) is caused by the degeneration of dopaminergic (DA) neurons in the substantia nigra (SN). Here, we established a method for voxel-based morphometry (VBM) and automatic tissue segmentation of the marmoset monkey brain using a 7-T animal scanner and applied the method to assess DA degeneration in a PD model, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated animals, with tyrosine-hydroxylase staining. The most significant decreases of local tissue volume were detected in the bilateral SN of MPTP-treated marmoset brains (-53.0% in right and -46.5% in left) and corresponded with the location of DA neurodegeneration found in histology (-65.4% in right). In addition to the SN, the decreases were also confirmed in the locus coeruleus, and lateral hypothalamus. VBM using 7-T MRI was effective in detecting volume loss in the SN of the PD-model marmoset. This study provides a potential basis for the application of VBM with ultra-high field MRI in the clinical diagnosis of PD. The developed method may also offer value in automatic whole-brain evaluation of structural changes for the marmoset monkey.

KEYWORDS:

Callithrix jacchus; MPTP; Parkinson’s disease; VBM; common marmoset; substantia nigra

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center