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J Fish Biol. 2015 Jul;87(1):43-53. doi: 10.1111/jfb.12690. Epub 2015 May 26.

Steelhead trout Oncorhynchus mykiss metabolic rate is affected by dietary Aloe vera inclusion but not by mounting an immune response against formalin-killed Aeromonas salmonicida.

Author information

1
Departamento de Fisiologia, Instituto de Biociências, Campus de Botucatu, UNESP, Universidade Estadual Paulista, Rubião Jr., 18618-970 SP, Brazil.
2
Centro de Aquicultura da Unesp, UNESP, Universidade Estadual Paulista, Via de Acesso Prof. Paulo Donato Castelane, 14.884-900, Jaboticabal, SP, Brazil.
3
Department of Ocean Sciences, Memorial University of Newfoundland, St John's, NL, A1C 5S7, Canada.

Abstract

The oxygen consumption (MO2) of two groups of 10° C acclimated steelhead trout Oncorhynchus mykiss was measured for 72 h after they were given a 100 µl kg(-1) intraperitoneal injection of formalin-killed Aeromonas salmonicida (ASAL) or phosphate-buffered saline (PBS). In addition, plasma cortisol levels were measured in fish from both groups prior to, and 1 and 3 h after, they were given a 30 s net stress. The first group was fed an unaltered commercial diet for 4 weeks, whereas the second group was fed the same diet but with 0·5% (5 g kg(-1) ) Aloe vera powder added; A. vera has potential as an immunostimulant for use in aquaculture, but its effects on basal and acute phase response (APR)-related metabolic expenditures and stress physiology, are unknown. Injection of ASAL v. PBS had no measurable effect on the MO2 of O. mykiss indicating that the APR in this species is not associated with any net increase in energy expenditure. In contrast, incorporating 0·5% A. vera powder into the feed decreased routine metabolic rate by c. 8% in both injection groups and standard metabolic rate in the ASAL-injected group (by c. 4 mg O2 kg(-1) h(-1) ; 5%). Aloe vera fed fish had resting cortisol levels that were approximately half of those in fish on the commercial diet (c. 2·5 v. 5·0 ng ml(-1) ), but neither this difference nor those post-stress reached statistical significance (P > 0·05).

KEYWORDS:

acute phase response; bacterial antigen; cortisol; immune function; immunostimulant; metabolic costs

PMID:
26010230
DOI:
10.1111/jfb.12690
[Indexed for MEDLINE]

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