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J Sep Sci. 2015 Aug;38(15):2630-9. doi: 10.1002/jssc.201500021. Epub 2015 Jul 3.

Quality control of processed Crataegi Fructus and its medicinal parts by ultra high performance liquid chromatography with electrospray ionization tandem mass spectrometry.

Yin F1, Li L1, Chen Y2, Lu T1, Li W1, Cai B1, Yin W1,3,4.

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College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, P. R. China.
Department of Chinese Medicine, Jiangsu Cancer Hospital, Nanjing, P. R. China.
The State Key Lab of Pharmaceutical Biotechnology, College of life Sciences, Nanjing University, Nanjing, China.
Jiangsu Key Laboratory of Pediatric Respiratory Disease, Nanjing University of Chinese Medicine, Nanjing 210023, China.


Crataegi Fructus, an edible food, has been used as a traditional medicine to treat diseases for many years. There is substantial evidence that multiple constituents are responsible for the beneficial effects of Crataegi Fructus. To effectively control the quality of this herbal medicine, we developed an ultra high performance liquid chromatography with electrospray ionization tandem mass spectrometry protocol to simultaneously quantify ten compounds (chlorogenic acid, procyanidin B2, l-epicatechin, glucosylvitexin, vitexin-2-O-rhamnoside, vitexin, rutin, hyperoside, isoquercitrin, and quercetin) in Crataegi Fructus. Multiple-reaction monitoring was used for the quantification in the negative mode for 8 min. This proposed method is simple, reliable, sensitive, and specific. Further, the quantification parameters, including linearity, limit of detection, limit of quantification, precision, reproducibility, stability, and accuracy were optimized. The quality of the processed samples of Crataegi Fructus was evaluated using this method. Additionally, the method was successfully used to distinguish the medicinal components, including peel, kernel, and flesh. The data described in this study offer valuable information for the quality control and proper use of Crataegi Fructus.


Crataegi Fructus; Multi-component analysis; Principal component analysis; Ultra high performance liquid chromatography


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