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Philos Trans R Soc Lond B Biol Sci. 2015 Jul 5;370(1672). pii: 20140193. doi: 10.1098/rstb.2014.0193.

Contribution of extrasynaptic N-methyl-D-aspartate and adenosine A1 receptors in the generation of dendritic glutamate-mediated plateau potentials.

Author information

1
Department of Neuroscience, University of Connecticut Health Center, Farmington, CT 06030, USA.
2
Department of Neuroscience, University of Connecticut Health Center, Farmington, CT 06030, USA antic@neuron.uchc.edu.

Abstract

Thin basal dendrites can strongly influence neuronal output via generation of dendritic spikes. It was recently postulated that glial processes actively support dendritic spikes by either ceasing glutamate uptake or by actively releasing glutamate and adenosine triphosphate (ATP). We used calcium imaging to study the role of NR2C/D-containing N-methyl-d-aspartate (NMDA) receptors and adenosine A1 receptors in the generation of dendritic NMDA spikes and plateau potentials in basal dendrites of layer 5 pyramidal neurons in the mouse prefrontal cortex. We found that NR2C/D glutamate receptor subunits contribute to the amplitude of synaptically evoked NMDA spikes. Dendritic calcium signals associated with glutamate-evoked dendritic plateau potentials were significantly shortened upon application of the NR2C/D receptor antagonist PPDA, suggesting that NR2C/D receptors prolong the duration of calcium influx during dendritic spiking. In contrast to NR2C/D receptors, adenosine A1 receptors act to abbreviate dendritic and somatic signals via the activation of dendritic K(+) current. This current is characterized as a slow-activating outward-rectifying voltage- and adenosine-gated current, insensitive to 4-aminopyridine but sensitive to TEA. Our data support the hypothesis that the release of glutamate and ATP from neurons or glia contribute to initiation, maintenance and termination of local dendritic glutamate-mediated regenerative potentials.

KEYWORDS:

calcium imaging; dendritic spike; neuromodulation; plateau potential

PMID:
26009772
PMCID:
PMC4455762
DOI:
10.1098/rstb.2014.0193
[Indexed for MEDLINE]
Free PMC Article

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