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Mol Biosyst. 2015 Jul;11(7):1820-3. doi: 10.1039/c5mb00326a.

A miniaturized readout strategy for endogenous histone deacetylase activity.

Author information

1
Interfaculty Institute of Biochemistry, University of Tuebingen, Hoppe-Seyler-Str. 4, 72076 Tuebingen, Germany. dirk.schwarzer@uni-tuebingen.de.

Abstract

Histone deacetylases are important drug targets, which are difficult to characterize due to their poor accessibility. We have developed a miniaturized assay for the multi-site readout of deacetylase activity and profiled the substrate selectivity of HDACs for acetylation sites on histone H4 and tumor suppressor protein p53.

PMID:
26009008
DOI:
10.1039/c5mb00326a
[Indexed for MEDLINE]

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