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J Exp Med. 2015 Jun 1;212(6):953-70. doi: 10.1084/jem.20150002. Epub 2015 May 25.

DYRK1A controls the transition from proliferation to quiescence during lymphoid development by destabilizing Cyclin D3.

Author information

1
Division of Hematology/Oncology, Northwestern University, Chicago, IL 60208.
2
Diaxonhit, 75013 Paris, France.
3
Normandie Université, COBRA, UMR 6014 and FR 3038; Université Rouen; INSA Rouen; Centre National de la Recherche Scientifique, Bâtiment IRCOF, 76821 Mont St. Aignan, France.
4
INSERM U985, Gustave Roussy, 94800 Villejuif, France.
5
Division of Hematology/Oncology, Northwestern University, Chicago, IL 60208 j-crispino@northwestern.edu.

Abstract

Pre-B and pre-T lymphocytes must orchestrate a transition from a highly proliferative state to a quiescent one during development. Cyclin D3 is essential for these cells' proliferation, but little is known about its posttranslational regulation at this stage. Here, we show that the dual specificity tyrosine-regulated kinase 1A (DYRK1A) restrains Cyclin D3 protein levels by phosphorylating T283 to induce its degradation. Loss of DYRK1A activity, via genetic inactivation or pharmacologic inhibition in mice, caused accumulation of Cyclin D3 protein, incomplete repression of E2F-mediated gene transcription, and failure to properly couple cell cycle exit with differentiation. Expression of a nonphosphorylatable Cyclin D3 T283A mutant recapitulated these defects, whereas inhibition of Cyclin D:CDK4/6 mitigated the effects of DYRK1A inhibition or loss. These data uncover a previously unknown role for DYRK1A in lymphopoiesis, and demonstrate how Cyclin D3 protein stability is negatively regulated during exit from the proliferative phases of B and T cell development.

PMID:
26008897
PMCID:
PMC4451127
DOI:
10.1084/jem.20150002
[Indexed for MEDLINE]
Free PMC Article

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