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Nat Methods. 2015 Jul;12(7):657-660. doi: 10.1038/nmeth.3410. Epub 2015 May 25.

Dexterous robotic manipulation of alert adult Drosophila for high-content experimentation.

Savall J#1,2,3, Ho ETW#1,4, Huang C1, Maxey JR1,3, Schnitzer MJ1,2,3.

Author information

1
James H. Clark Center, Stanford University, Stanford, California, USA.
2
Howard Hughes Medical Institute, Stanford University, Stanford, California, USA.
3
CNC Program, Stanford University, Stanford, California, USA.
4
Centre for Intelligent Signal and Imaging Research, Universiti Teknologi Petronas, Perak, Malaysia.
#
Contributed equally

Abstract

We present a robot that enables high-content studies of alert adult Drosophila by combining operations including gentle picking; translations and rotations; characterizations of fly phenotypes and behaviors; microdissection; or release. To illustrate, we assessed fly morphology, tracked odor-evoked locomotion, sorted flies by sex, and dissected the cuticle to image neural activity. The robot's tireless capacity for precise manipulations enables a scalable platform for screening flies' complex attributes and behavioral patterns.

PMID:
26005812
PMCID:
PMC4490062
DOI:
10.1038/nmeth.3410
[Indexed for MEDLINE]
Free PMC Article

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