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Dev Cell. 2015 Jun 8;33(5):522-34. doi: 10.1016/j.devcel.2015.03.024. Epub 2015 May 21.

Mitotic Control of Planar Cell Polarity by Polo-like Kinase 1.

Author information

1
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
2
Department of Chemistry, Princeton University, Princeton, NJ 08544, USA.
3
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA. Electronic address: danelle@princeton.edu.

Abstract

During cell division, polarized epithelial cells employ mechanisms to preserve cell polarity and tissue integrity. In dividing cells of the mammalian skin, planar cell polarity (PCP) is maintained through the bulk internalization, equal segregation, and polarized recycling of cortical PCP proteins. The dramatic redistribution of PCP proteins coincides precisely with cell-cycle progression, but the mechanisms coordinating PCP and mitosis are unknown. Here we identify Plk1 as a master regulator of PCP dynamics during mitosis. Plk1 interacts with core PCP component Celsr1 via a conserved polo-box domain (PBD)-binding motif, localizes to mitotic endosomes, and directly phosphorylates Celsr1. Plk1-dependent phosphorylation activates the endocytic motif specifically during mitosis, allowing bulk recruitment of Celsr1 into endosomes. Inhibiting Plk1 activity blocks PCP internalization and perturbs PCP asymmetry. Mimicking dileucine motif phosphorylation is sufficient to drive Celsr1 internalization during interphase. Thus, Plk1-mediated phosphorylation of Celsr1 ensures that PCP redistribution is precisely coordinated with mitotic entry.

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PMID:
26004507
PMCID:
PMC4464975
DOI:
10.1016/j.devcel.2015.03.024
[Indexed for MEDLINE]
Free PMC Article

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