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Cell Tissue Res. 2015 Nov;362(2):295-305. doi: 10.1007/s00441-015-2199-3. Epub 2015 May 24.

Expression and localization of the diacylglycerol kinase family and of phosphoinositide signaling molecules in adrenal gland.

Author information

1
Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Iida-nishi 2-2-2, Yamagata, 990-9585, Japan. yahodumi@med.id.yamagata-u.ac.jp.
2
Department of Anatomy and Cell Biology, Yamagata University School of Medicine, Iida-nishi 2-2-2, Yamagata, 990-9585, Japan.
3
Department of Psychiatry, Yamagata University School of Medicine, Yamagata, 990-9585, Japan.
4
Department of Anatomy, Hokkaido University Graduate School of Medicine, Sapporo, 060-8638, Japan.

Abstract

Adrenal glands play a central role in the secretion of steroid hormones and catecholamines. Previous studies have revealed that molecules engaged in phosphoinositide (PI) turnover are expressed in the adrenal gland, suggesting the importance of PI signaling in adrenal signal transduction. Diacylglycerol kinase (DGK) catalyzes the phosphorylation of diacylglycerol (DG), a major second messenger in the PI signaling cascade. The DGK family is expressed in distinct patterns in endocrine organs at the mRNA and protein levels. Nevertheless, little is known about the characteristics and morphological aspects of DGKs in the adrenal gland. We have performed immunohistochemical analyses to investigate the expression and localization of DGK isozymes, together with PI signaling molecules, in the adrenal gland at the protein level. Our results show that the DGK family and a set of PI signaling molecules are expressed intensely in zona glomerulosa cells and medullary chromaffin cells in the adrenal gland. In adrenal cells, DGKγ localizes to the Golgi complex, DGKε to the plasma membrane, and DGKζ to the nucleus. These findings show the distinct expression and subcellular localization of DGK isozymes and PI signaling molecules in the adrenal gland, suggesting that each DGK isozyme has a role in signal transduction in adrenal cells, especially in the zona glomerulosa and medulla.

KEYWORDS:

Diacylglycerol kinase; Immunohistochemistry; Medulla; Phosphoinositide turnover; Rat (Wistar, male); Zona glomerulosa

PMID:
26003177
DOI:
10.1007/s00441-015-2199-3
[Indexed for MEDLINE]

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