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Tumour Biol. 2015 Nov;36(11):8309-16. doi: 10.1007/s13277-015-3582-0. Epub 2015 May 23.

miR-582-5p inhibits proliferation of hepatocellular carcinoma by targeting CDK1 and AKT3.

Author information

1
Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Wuhan, China.
2
Oncology Department of the First People's Hospital of Shunde, Guangzhou, China.
3
Anesthesia Department, Zhongnan Hospital of Wuhan University, Wuhan, China.
4
Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Wuhan, China. yqf_china@163.com.
5
Research Center of National Health Ministry on Transplantation Medicine Engineering and Technology, The 3rd Xiangya Hospital of Central South University, Changsha, China. yqf_china@163.com.
6
Zhongnan Hospital of Wuhan University, Donghu Road 169#, Wuchang District, Wuhan, 430071, China. yqf_china@163.com.

Abstract

microRNAs play an important role in the progression of hepatocellular carcinoma (HCC). In this study, we found that miR-582-5p expression was downregulated in hepatoma tissues and HCC cell lines. Upregulation of miR-582-5p reduced colony number, inhibited cellular proliferation, and arrested cell cycle in G0/G1 phase. When miR-582-5p was inhibited, the colony number was increased and cellular proliferation and cell cycle were promoted. Further studies showed that miR-582-5p regulated the progression of HCC through directly inhibiting the expression of CDK1 and AKT3, and indirectly inhibiting the expression of cyclinD1.

KEYWORDS:

AKT3; CDK1; CyclinD1; Hepatocellular carcinoma; miR-582-5p

PMID:
26002580
DOI:
10.1007/s13277-015-3582-0
[Indexed for MEDLINE]

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