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Anal Chim Acta. 2015 Jun 16;879:97-103. doi: 10.1016/j.aca.2015.03.036. Epub 2015 Mar 26.

Fluorescent probe for turn-on sensing of L-cysteine by ensemble of AuNCs and polymer protected AuNPs.

Author information

1
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, No. 2 Zhongguancun Beiyijie, Beijing 100190, PR China; College of Chemistry, Tianjin Normal University, No. 393, Extension of Bin Shui West Road, Xiqing District, Tianjin 300387, PR China.
2
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, No. 2 Zhongguancun Beiyijie, Beijing 100190, PR China. Electronic address: qiaojuan@iccas.ac.cn.
3
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, No. 2 Zhongguancun Beiyijie, Beijing 100190, PR China; Graduate School, University of Chinese Academy of Sciences, 19A Yuquanlu, Beijing 100049, PR China.
4
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, No. 2 Zhongguancun Beiyijie, Beijing 100190, PR China. Electronic address: qili@iccas.ac.cn.
5
College of Chemistry, Tianjin Normal University, No. 393, Extension of Bin Shui West Road, Xiqing District, Tianjin 300387, PR China.

Abstract

A new fluorescent probe based on ensemble of gold nanoclusters (AuNCs) and polymer protected gold nanoparticles (AuNPs) for turn-on sensing of L-cysteine was designed and prepared. The AuNCs were protected by bovine serum albumin and had strong fluorescence. The polymer protected AuNPs were synthesized by a facile in situ strategy at room temperature and could quench the fluorescence of AuNCs due to the Förster resonance energy transfer. Interestingly, it has been observed that the quenched fluorescence of AuNCs was recovered by L-cysteine, which could induce the aggregation of polymer protected AuNPs by sulfur group. Then the prepared fluorescent probe was successfully used for determination of L-Cys in human urines, which would have an evolving aspect and promote the subsequent exploration.

KEYWORDS:

Determination of l-cysteine; Fluorescent turn-on sensing; Gold nanoclusters; Polymer protected gold nanoparticles

PMID:
26002483
DOI:
10.1016/j.aca.2015.03.036
[Indexed for MEDLINE]

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