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Biochem Biophys Res Commun. 2015 Aug 7;463(4):483-9. doi: 10.1016/j.bbrc.2015.04.151. Epub 2015 May 20.

Drosophila p24 and Sec22 regulate Wingless trafficking in the early secretory pathway.

Author information

1
State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
2
State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
3
School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China.
4
Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA.
5
State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA. Electronic address: xinhua.lin@ioz.ac.cn.

Abstract

The Wnt signaling pathway is crucial for development and disease. The regulation of Wnt protein trafficking is one of the pivotal issues in the Wnt research field. Here we performed a genetic screen in Drosophila melanogaster for genes involved in Wingless/Wnt secretion, and identified the p24 protein family members Baiser, CHOp24, Eclair and a v-SNARE protein Sec22, which are involved in the early secretory pathway of Wingless/Wnt. We provided genetic evidence demonstrating that loss of p24 proteins or Sec22 impedes Wingless (Wg) secretion in Drosophila wing imaginal discs. We found that Baiser cannot replace other p24 proteins (CHOp24 or Eclair) in escorting Wg, and only Baiser and CHOp24 interact with Wg. Moreover, we showed that the v-SNARE protein Sec22 and Wg are packaged together with p24 proteins. Taken together, our data provide important insights into the early secretory pathway of Wg/Wnt.

KEYWORDS:

Baiser; Early secretory pathway; Sec22; Wingless/Wnt trafficking; p24

PMID:
26002470
PMCID:
PMC4764680
DOI:
10.1016/j.bbrc.2015.04.151
[Indexed for MEDLINE]
Free PMC Article

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