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Biochem Biophys Res Commun. 2015 Jul 17-24;463(1-2):130-6. doi: 10.1016/j.bbrc.2015.05.047. Epub 2015 May 20.

Transforming growth factor β-activated kinase 1 negatively regulates interleukin-1α-induced stromal-derived factor-1 expression in vascular smooth muscle cells.

Author information

1
Department of Hepatobiliary Surgery, Union Hospital, Tongji Medical College, Huangzhong University of Science and Technology, Wuhan 430022, China.
2
Department of Gerontology, Union Hospital, Tongji Medical College, Huangzhong University of Science and Technology, Wuhan 430022, China.
3
Department of Hepatobiliary Pancreatic Surgery, People's Hospital of Zhengzhou University, School of Medicine, Zhengzhou University, Zhengzhou 450003, China.
4
Department of Hepatobiliary Surgery, The Second Hospital, Hebei Medical University, Shijiazhuang 050000, China. Electronic address: sanguang1998@sina.com.
5
Department of Hepatobiliary Surgery, Union Hospital, Tongji Medical College, Huangzhong University of Science and Technology, Wuhan 430022, China. Electronic address: zsong@hust.edu.cn.

Abstract

Stromal-derived Factor-1 (SDF-1) derived from vascular smooth muscle cells (VSMCs) contributes to vascular repair and remodeling in various vascular diseases. In this study, the mechanism underlying regulation of SDF-1 expression by interleukin-1α (IL-1α) was investigated in primary rat VSMCs. We found IL-1α promotes SDF-1 expression by up-regulating CCAAT-enhancer-binding protein β (C/EBPβ) in an IκB kinase β (IKKβ) signaling-dependent manner. Moreover, IL-1α-induced expression of C/EBPβ and SDF-1 was significantly potentiated by knockdown of transforming growth factor β-activated kinase 1 (TAK1), an upstream activator of IKKβ signaling. In addition, we also demonstrated that TAK1/p38 mitogen-activated protein kinase (p38 MAPK) signaling exerted negative effect on IL-1α-induced expression of C/EBPβ and SDF-1 through counteracting ROS-dependent up-regulation of nuclear factor erythroid 2-related factor 2 (NRF2). In conclusion, TAK1 acts as an important regulator of IL-1α-induced SDF-1 expression in VSMCs, and modulating activity of TAK1 may serve as a potential strategy for modulating vascular repair and remodeling.

KEYWORDS:

CCAAT-enhancer-binding protein β; Interleukin-1; Nuclear factor erythroid 2-related factor 2; Stromal-derived factor-1; Transforming growth factor β-activated kinase 1; p38 mitogen-activated protein kinase

PMID:
26002466
DOI:
10.1016/j.bbrc.2015.05.047
[Indexed for MEDLINE]

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