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PLoS Genet. 2015 May 22;11(5):e1005240. doi: 10.1371/journal.pgen.1005240. eCollection 2015 May.

Parp3 negatively regulates immunoglobulin class switch recombination.

Author information

1
Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France; Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France; Centre National de Recherche Scientifique, UMR7104, Illkirch, France; Université de Strasbourg, Illkirch, France.
2
Centre National de Recherche Scientifique, UMR7242, Illkirch, France; Université de Strasbourg, Laboratoire d'Excellence Medalis, Illkirch, France; Institut de Recherche de l'Ecole de Biotechnologie de Strasbourg, Illkirch, France; Ecole Supérieure de Biotechnologie de Strasbourg, Illkirch, France.

Abstract

To generate highly specific and adapted immune responses, B cells diversify their antibody repertoire through mechanisms involving the generation of programmed DNA damage. Somatic hypermutation (SHM) and class switch recombination (CSR) are initiated by the recruitment of activation-induced cytidine deaminase (AID) to immunoglobulin loci and by the subsequent generation of DNA lesions, which are differentially processed to mutations during SHM or to double-stranded DNA break intermediates during CSR. The latter activate the DNA damage response and mobilize multiple DNA repair factors, including Parp1 and Parp2, to promote DNA repair and long-range recombination. We examined the contribution of Parp3 in CSR and SHM. We find that deficiency in Parp3 results in enhanced CSR, while SHM remains unaffected. Mechanistically, this is due to increased occupancy of AID at the donor (Sμ) switch region. We also find evidence of increased levels of DNA damage at switch region junctions and a bias towards alternative end joining in the absence of Parp3. We propose that Parp3 plays a CSR-specific role by controlling AID levels at switch regions during CSR.

PMID:
26000965
PMCID:
PMC4441492
DOI:
10.1371/journal.pgen.1005240
[Indexed for MEDLINE]
Free PMC Article

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