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Mol Cell. 2015 May 21;58(4):575-85. doi: 10.1016/j.molcel.2015.04.028.

Choosing the Right Tool for the Job: RNAi, TALEN, or CRISPR.

Author information

1
Department of Microbiology and Immunology, UCSF Diabetes Center, Keck Center for Noncoding RNA, University of California, San Francisco, San Francisco, CA 94143, USA.
2
Department of Microbiology and Immunology, UCSF Diabetes Center, Keck Center for Noncoding RNA, University of California, San Francisco, San Francisco, CA 94143, USA. Electronic address: michael.mcmanus@ucsf.edu.

Abstract

The most widely used approach for defining gene function is to reduce or completely disrupt its normal expression. For over a decade, RNAi has ruled the lab, offering a magic bullet to disrupt gene expression in many organisms. However, new biotechnological tools--specifically CRISPR-based technologies--have become available and are squeezing out RNAi dominance in mammalian cell studies. These seemingly competing technologies leave research investigators with the question: "Which technology should I use in my experiment?" This review offers a practical resource to compare and contrast these technologies, guiding the investigator when and where to use this fantastic array of powerful tools.

PMID:
26000843
PMCID:
PMC4441801
DOI:
10.1016/j.molcel.2015.04.028
[Indexed for MEDLINE]
Free PMC Article

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