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FEMS Microbiol Ecol. 2015 Jun;91(6). pii: fiv054. doi: 10.1093/femsec/fiv054. Epub 2015 May 20.

Interindividual differences in response to treatment with butyrate-producing Butyricicoccus pullicaecorum 25-3T studied in an in vitro gut model.

Author information

1
Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, 9000 Ghent, Belgium.
2
Laboratory of Molecular Bacteriology (Rega Institute), KU Leuven, O&N I Herestraat 49, 3000 Leuven, Belgium VIB Center for the Biology of Disease, Herestraat 49, B-3000 Leuven, Belgium.
3
Department of Pathology, Bacteriology and Avian Diseases, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
4
Department of Gastroenterology, Ghent University, 9000 Ghent, Belgium.
5
Laboratory of Molecular Bacteriology (Rega Institute), KU Leuven, O&N I Herestraat 49, 3000 Leuven, Belgium VIB Center for the Biology of Disease, Herestraat 49, B-3000 Leuven, Belgium Microbiology Unit, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium.
6
Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, 9000 Ghent, Belgium tom.vandewiele@ugent.be.

Abstract

Butyrate-producing bacteria are promising probiotic candidates to target microbial dysbiosis in gastrointestinal disorders like inflammatory bowel diseases. Butyricicoccus pullicaecorum 25-3(T), a butyrate-producing clostridial cluster IV strain, is such a candidate. Little is known about its abundance in the colon microbiota and its butyrogenic properties. We used the M-SHIME(®), an in vitro simulator for the human intestinal microbial ecosystem, to study the effect of supplementing a single dose of B. pullicaecorum 25-3(T) on lumen- and mucus-associated microbiota of eight individuals. Butyricicoccus pullicaecorum was more abundant in mucus-associated microbiota compared with lumen microbiota. Supplementation with a single dose of B. pullicaecorum 25-3(T) resulted in a temporary increase in B. pullicaecorum bacteria in lumen compartment of all individuals. In two cases, the responders, an increased butyrate production was observed as compared with the control. 16S rRNA gene amplicon sequencing revealed the microbiota of responders to be different as compared to non-responder microbiota. We can conclude that B. pullicaecorum 25-3(T) is a mucus-associated bacterium whose potency to stimulate butyrate production is characterized by a large interindividual variability in terms of composition of the receiving microbial community.

KEYWORDS:

anaerobe; butyric acid; colonization; gastrointestinal microbial ecology; in vitro; probiotic

PMID:
25999470
DOI:
10.1093/femsec/fiv054
[Indexed for MEDLINE]

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