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Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.

A fluorescent tool set for yeast Atg proteins.

Author information

1
a School of Life Sciences and Biotechnology; Shanghai Jiao Tong University ; Shanghai , China.

Abstract

Fluorescence microscopy of live cells is instrumental in deciphering the molecular details of autophagy. To facilitate the routine examination of yeast Atg proteins under diverse conditions, here we provide a comprehensive tool set, including (1) plasmids for the expression of GFP chimeras at endogenous levels for most Atg proteins, (2) RFP-Atg8 constructs with improved properties as a PAS marker, and (3) plasmids for the complementation of common yeast auxotrophic markers. We hope that the availability of this tool set will further accelerate yeast autophagy research.

KEYWORDS:

Atg, autophagy related; C,G,R,YFP, cyan, green, red and yellow fluorescent protein; Cvt, cytoplasm-to-vacuole targeting; DsRed eExpress 2; PAS, phagophore assembly site; Vps, vacuolar protein sorting.; autophagy; auxotroph; fluorescent protein; mKO, monomeric Kusabira Orange; pseudo-monomer; starter kit; yeast

PMID:
25998947
PMCID:
PMC4502664
DOI:
10.1080/15548627.2015.1040971
[Indexed for MEDLINE]
Free PMC Article

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