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J Appl Physiol (1985). 2015 Jul 15;119(2):124-34. doi: 10.1152/japplphysiol.00077.2015. Epub 2015 May 21.

Circulating inflammatory miRNA signature in response to different doses of aerobic exercise.

Author information

1
Lipids and Cardiovascular Pathology Group, Research Institute of the Sant Pau Hospital (IIB Sant Pau), Barcelona, Spain; Department of Functional Biology (Physiology), University of Oviedo, Oviedo, Spain; david.degonzalo@gmail.com.
2
Laboratory of Disorders of Lipid Metabolism and Molecular Nutrition, Madrid Institute for Advanced Studies-Food, Campus de Excelencia Internacional Autonomous University of Madrid and Spanish National Research Council, Madrid, Spain;
3
Department of Pharmaceutical and Health Sciences, Centro de Estudios Universitarios San Pablo University, Madrid, Spain;
4
Hospital Universitario Central de Asturias, Hospital Univeritario Central de Asturias, Asturias, Spain; Universidad Autónoma de Chile, Santiago, Chile; and.
5
Sports Medicine Center, Clinical Laboratory, Higher Council for Sports, Madrid, Spain.
6
Department of Functional Biology (Physiology), University of Oviedo, Oviedo, Spain; Department of Pharmaceutical and Health Sciences, Centro de Estudios Universitarios San Pablo University, Madrid, Spain;

Abstract

While moderate acute exercise has been associated with strong anti-inflammatory mechanisms, strenuous exercise has been linked to deleterious inflammatory perturbations. It is therefore fundamental to elucidate the mechanisms that regulate the exercise-induced inflammatory cascade. Information on novel regulators such as circulating inflammatory microRNAs (c-inflammamiRs) is incomplete. In this study, we evaluated the response of a panel of c-inflammamiRs to different doses of acute aerobic exercise. We first studied the exercise-induced inflammatory cascade in serum samples of nine active middle-aged males immediately before and after (0 h, 24 h, 72 h) 10-km, half-marathon, and marathon races. Next, we analyzed the circulating profile of 106 specific c-inflammamiRs immediately before) and after (0 h, 24 h) 10-km (low inflammatory response) and marathon (high inflammatory response) races. Analysis of classical inflammatory parameters revealed a dose-dependent effect of aerobic exercise on systemic inflammation, with higher levels detected after marathon. We observed an increase in miR-150-5p immediately after the 10-km race. Levels of 12 c-inflammamiRs were increased immediately after the marathon (let-7d-3p, let-7f-2-3p, miR-125b-5p, miR-132-3p, miR-143-3p, miR-148a-3p, miR-223-3p, miR-223-5p, miR-29a-3p, miR-34a-5p, miR-424-3p, and miR-424-5p). c-inflammamiRs returned to basal levels after 24 h. Correlation and in silico analyses supported a close association between the observed c-inflammamiR pattern and regulation of the inflammatory process. In conclusion, we found that different doses of acute aerobic exercise induced a distinct and specific c-inflammamiR response, which may be associated with control of the exercise-induced inflammatory cascade. Our findings point to c-inflammamiRs as potential biomarkers of exercise-induced inflammation, and hence, exercise dose.

KEYWORDS:

circulating microRNAs; exercise; inflammation

PMID:
25997943
DOI:
10.1152/japplphysiol.00077.2015
[Indexed for MEDLINE]
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