Format

Send to

Choose Destination
Methods Cell Biol. 2015;128:165-200. doi: 10.1016/bs.mcb.2015.02.007. Epub 2015 Apr 8.

Structural and functional studies of membrane remodeling machines.

Author information

1
Department of Biochemistry, University of Utah, School of Medicine, Salt Lake City, UT, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, USA.

Abstract

Building cells from their component parts will hinge upon our ability to reconstitute biochemical compartmentalization and exchange between membrane-delimited organelles. By contrast with our understanding of other cellular events, the mechanisms that govern membrane trafficking has lagged because the presence of phospholipid bilayers complicates the use of standard methods. This chapter describes in vitro methods for purifying, reconstituting, and visualizing membrane remodeling activities directly by electron cryomicroscopy.

KEYWORDS:

Bilayer; Dynamin; Electron cryomicroscopy; Endocytosis; Endophilin; Lipid-binding; Membrane remodeling; Mitochondria; cryo-EM

PMID:
25997348
DOI:
10.1016/bs.mcb.2015.02.007
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center