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Wei Sheng Yan Jiu. 2015 Mar;44(2):185-9, 195.

[Effects of biotin on blood glucose regulation in type 2 diabetes rat model].

[Article in Chinese]

Abstract

OBJECTIVE:

To explore the effect of biotin on blood glucose regulation in rats and its possible mechanism.

METHODS:

According to initial body weight and blood glucose, we randomly divided the 90 Wistar rats into 5 groups: the normal control group, model group, biotin low-dose group (0. 6mg/kg BW), biotin medium-dose group (3. 0 mg/kg BW) and biotin high-dose group (6. 0 mg/kg BW). After 2 months, the rats with HFS feed were injected with STZ (25 mg/kg BW) to manufacture diabetic rat model. After the OGTT experiment at 10th week, the blood glucose, insulin, liver/muscle glycogen and other biochemical indexes were detected. The GCK, PCK1 mRNA expression were measured with RT-PCR method.

RESULTS:

Biotin has a certain improvement on postprandial glucose in diabetic rats. Compared with the model group, the AUC and the 30min postprandial blood glucose of biotin high-dose group were significantly decreased (P <0. 05). Biotin can affect some key enzyme gene in glucose metabolism, such as GCK, PCK1.

CONCLUSION:

The possible mechanism of the decreasing biotin blood sugar in diabetic rats may by promoting the synthesis of glycogenand reducing gluconeogenesis.

PMID:
25997216
[Indexed for MEDLINE]

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