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Rev Col Bras Cir. 2015 Jan-Feb;42(1):43-8. doi: 10.1590/0100-69912015001009.

Preliminary study of coconut water for graft tissues preservation in transplantation.

[Article in English, Portuguese]

Author information

1
Faculty of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
2
Department Surgery, Faculty of Medicine, UFMG, Minas Gerais, Brazil.
3
Department of Complementary Assessment, Faculty of Medicine, UFMG, Minas Gerais, Brazil.
4
Faculty of Medicine, UFMG, Minas Gerais, Brazil.
5
Department of Pathology, Faculty of Medicine, UFMG, Minas Gerais, Brazil.

Abstract

OBJECTIVE:

to verify the effectiveness of coconut water in preserving tissues for transplant.

METHODS:

Fifty male Wistar rats were randomly distributed in five groups, according to the following preservation solutions for tissue grafts: Group 1: Lactated Ringer; Group 2: Belzer solution; Group 3: mature coconut water; Group 4: green coconut water; Group 5: modified coconut water. In Group 5, the green coconut water has been modified like the Belzer solution. From each animal we harvested the spleen, ovaries and skin of the back segment. These tissues were preserved for six hours in one of the solutions. Then, the grafts were reimplanted. The recovery of the function of the implanted tissues was assessed 90 days after surgery, by splenic scintigraphy and blood exam. The implanted tissues were collected for histopathological examination.

RESULTS:

The serum levels did not differ among groups, except for the animals in Group 5, which showed higher levels of IgG than Group 1, and differences in relation to FSH between groups 1 and 2 (p <0.001), 4 and 2 (p = 0.03) and 5 and 2 (p = 0.01). The splenic scintigraphy was not different between groups. The ovarian tissue was better preserved in mature coconut water (p <0.007).

CONCLUSION:

the coconut water-based solutions preserves spleen, ovary, and rat skin for six hours, maintaining their normal function.

PMID:
25992700
DOI:
10.1590/0100-69912015001009
[Indexed for MEDLINE]
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