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PLoS One. 2015 May 18;10(5):e0126823. doi: 10.1371/journal.pone.0126823. eCollection 2015.

Characterization of the proteome of cytoplasmic lipid droplets in mouse enterocytes after a dietary fat challenge.

Author information

1
Department of Nutrition Science, Purdue University, West Lafayette, Indiana, United States of America.
2
Department of Biological Sciences, Purdue University, West Lafayette, Indiana, United States of America; Bindley Bioscience Center, Purdue University, West Lafayette, Indiana, United States of America.
3
Department of Biological Sciences, Purdue University, West Lafayette, Indiana, United States of America; Department of Entomology, Purdue University, West Lafayette, Indiana, United States of America.
4
Bindley Bioscience Center, Purdue University, West Lafayette, Indiana, United States of America.
5
Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, Massachusetts, United States of America.

Abstract

Dietary fat absorption by the small intestine is a multistep process that regulates the uptake and delivery of essential nutrients and energy. One step of this process is the temporary storage of dietary fat in cytoplasmic lipid droplets (CLDs). The storage and mobilization of dietary fat is thought to be regulated by proteins that associate with the CLD; however, mechanistic details of this process are currently unknown. In this study we analyzed the proteome of CLDs isolated from enterocytes harvested from the small intestine of mice following a dietary fat challenge. In this analysis we identified 181 proteins associated with the CLD fraction, of which 37 are associated with known lipid related metabolic pathways. We confirmed the localization of several of these proteins on or around the CLD through confocal and electron microscopy, including perilipin 3, apolipoprotein A-IV, and acyl-CoA synthetase long-chain family member 5. The identification of the enterocyte CLD proteome provides new insight into potential regulators of CLD metabolism and the process of dietary fat absorption.

PMID:
25992653
PMCID:
PMC4436333
DOI:
10.1371/journal.pone.0126823
[Indexed for MEDLINE]
Free PMC Article

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