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Plant Cell. 2015 Jun;27(6):1670-80. doi: 10.1105/tpc.15.00091. Epub 2015 May 19.

The Arabidopsis SWI2/SNF2 Chromatin Remodeling ATPase BRAHMA Targets Directly to PINs and Is Required for Root Stem Cell Niche Maintenance.

Author information

1
Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan.
2
Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, London, Ontario N5V 4T3, Canada Department of Biology, Western University, London, Ontario N6A 5B7, Canada.
3
Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
4
College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
5
Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan.
6
Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, London, Ontario N5V 4T3, Canada.
7
Guangdong Key Lab of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, China.
8
Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan kewu@ntu.edu.tw.

Abstract

BRAHMA (BRM), a SWI/SNF chromatin remodeling ATPase, is essential for the transcriptional reprogramming associated with development and cell differentiation in Arabidopsis thaliana. In this study, we show that loss-of-function mutations in BRM led to defective maintenance of the root stem cell niche, decreased meristematic activity, and stunted root growth. Mutations of BRM affected auxin distribution by reducing local expression of several PIN-FORMED (PIN) genes in the stem cells and impaired the expression of the stem cell transcription factor genes PLETHORA (PLT1) and PLT2. Chromatin immunoprecipitation assays showed that BRM could directly target to the chromatin of PIN1, PIN2, PIN3, PIN4, and PIN7. In addition, genetic interaction assays indicate that PLTs acted downstream of BRM, and overexpression of PLT2 partially rescued the stem cell niche defect of brm mutants. Taken together, these results support the idea that BRM acts in the PLT pathway to maintain the root stem cell niche by altering the expression of PINs.

PMID:
25991732
PMCID:
PMC4498203
DOI:
10.1105/tpc.15.00091
[Indexed for MEDLINE]
Free PMC Article

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