Format

Send to

Choose Destination
Biomaterials. 2015 Aug;60:121-9. doi: 10.1016/j.biomaterials.2015.04.061. Epub 2015 May 15.

Availability of extracellular matrix biopolymers and differentiation state of human mesenchymal stem cells determine tissue-like growth in vitro.

Author information

1
Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials, Dresden, Germany.
2
Max Planck Institute of Colloids and Interfaces, Department of Biomaterials, Potsdam, Germany.
3
Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials, Dresden, Germany; Center for Regenerative Therapies Dresden, TU Dresden, Dresden, Germany. Electronic address: werner@ipfdd.de.

Abstract

To explore the space-filling growth of adherent mesenchymal stem cells (MSC) into tissue-like structures in vitro, human bone marrow derived MSC were exposed to fibronectin-coated, millimeter-sized, triangular channels casted in poly(dimethyl siloxane) carriers. The results revealed that the three dimensional (3D) growth of MSC differs in dependence on differentiation status and availability of extracellular matrix (ECM) proteins: Massive 3D structure formation was observed for MSC under pro-osteogenic stimulation but not for undifferentiated MSC nor for MSC under pro-adipogenic stimulation; boosting cellular matrix secretion and addition of soluble ECM proteins caused extensive 3D tissue formation of undifferentiated MSC. The reported findings may contribute to bridge the gap between in vitro and in vivo analyses and guide the application of MSC in tissue replacement approaches.

KEYWORDS:

Extracellular matrix; Mesenchymal stem cells; Three-dimensional growth

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center