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Dev Growth Differ. 2015 Jun;57(5):369-377. doi: 10.1111/dgd.12216. Epub 2015 May 19.

A transportable, inexpensive electroporator for in utero electroporation.

Author information

1
Frey Initiative Research Unit, RIKEN Quantitative Biology Center, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
2
Laboratory for Neocortical Development, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
3
Department of Molecular and Cellular Mechanisms of Neurodegeneration, Paul Flechsig Institute of Brain Research, University of Leipzig, Liebigstraβe 19, 04103, Leipzig, Germany.

Abstract

Electroporation is a useful technique to study gene function during development but its broad application is hampered due to the expensive equipment needed. We describe the construction of a transportable, simple and inexpensive electroporator delivering square pulses with varying length and amplitude. The device was successfully used for in utero electroporation in mouse with a performance comparable to that of commercial products.

KEYWORDS:

cortex; electronic circuit; electroporation; hippocampus

PMID:
25988525
DOI:
10.1111/dgd.12216

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