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J Cell Biol. 2015 May 25;209(4):549-62. doi: 10.1083/jcb.201412111. Epub 2015 May 18.

The Msd1-Wdr8-Pkl1 complex anchors microtubule minus ends to fission yeast spindle pole bodies.

Author information

1
The Francis Crick Institute, Lincoln's Inn Fields Laboratory, London WC2A 3LY, England, UK Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima 739-8530, Japan.
2
The Francis Crick Institute, Lincoln's Inn Fields Laboratory, London WC2A 3LY, England, UK.
3
The Francis Crick Institute, Lincoln's Inn Fields Laboratory, London WC2A 3LY, England, UK takashi.toda@crick.ac.uk.

Abstract

The minus ends of spindle microtubules are anchored to a microtubule-organizing center. The conserved Msd1/SSX2IP proteins are localized to the spindle pole body (SPB) and the centrosome in fission yeast and humans, respectively, and play a critical role in microtubule anchoring. In this paper, we show that fission yeast Msd1 forms a ternary complex with another conserved protein, Wdr8, and the minus end-directed Pkl1/kinesin-14. Individual deletion mutants displayed the identical spindle-protrusion phenotypes. Msd1 and Wdr8 were delivered by Pkl1 to mitotic SPBs, where Pkl1 was tethered through Msd1-Wdr8. The spindle-anchoring defect imposed by msd1/wdr8/pkl1 deletions was suppressed by a mutation of the plus end-directed Cut7/kinesin-5, which was shown to be mutual. Intriguingly, Pkl1 motor activity was not required for its anchoring role once targeted to the SPB. Therefore, spindle anchoring through Msd1-Wdr8-Pkl1 is crucial for balancing the Cut7/kinesin-5-mediated outward force at the SPB. Our analysis provides mechanistic insight into the spatiotemporal regulation of two opposing kinesins to ensure mitotic spindle bipolarity.

PMID:
25987607
PMCID:
PMC4442821
DOI:
10.1083/jcb.201412111
[Indexed for MEDLINE]
Free PMC Article

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