Confirmation of the absence of tetrodotoxin and its analogues in the juveniles of the Japanese fire-bellied newt, Cynops pyrrhogaster, captive-reared from eggs in the laboratory using HILIC-LC-MS

Toxicon. 2015 Jul:101:101-5. doi: 10.1016/j.toxicon.2015.05.008. Epub 2015 May 16.

Abstract

The tetrodotoxin (TTX) contents of the Japanese fire-bellied newt, Cynops pyrrhogaster, captive-reared from eggs to metamorphosed juveniles with a non-toxic diet for 70 weeks, as well as wild-caught juvenile newts, were investigated using a high-resolution hydrophilic interaction chromatography-LC-MS. TTX was detected in 0- to 22-week-old captive-reared juvenile newts but was not detected (<15 ng/g) in the 36- to 70-week-old newts, while significant levels of TTX (1.3-14 μg/g) were detected in the wild-caught juveniles.

Keywords: Cynops pyrrhogaster; LC-MS; Newt; Tetrodotoxin; Toxicity analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatography, Liquid / methods*
  • Female
  • Mass Spectrometry / methods*
  • Metamorphosis, Biological / drug effects
  • Ovum / drug effects*
  • Salamandridae / metabolism*
  • Tetrodotoxin / analysis*

Substances

  • Tetrodotoxin