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ACS Appl Mater Interfaces. 2015 Jun 10;7(22):12249-53. doi: 10.1021/acsami.5b02744. Epub 2015 May 28.

Analyzing Carbohydrate-Protein Interaction Based on Single Plasmonic Nanoparticle by Conventional Dark Field Microscopy.

Abstract

We demonstrated a practical method to analyze carbohydrate-protein interaction based on single plasmonic nanoparticles by conventional dark field microscopy (DFM). Protein concanavalin A (ConA) was modified on large sized gold nanoparticles (AuNPs), and dextran was conjugated on small sized AuNPs. As the interaction between ConA and dextran resulted in two kinds of gold nanoparticles coupled together, which caused coupling of plasmonic oscillations, apparent color changes (from green to yellow) of the single AuNPs were observed through DFM. Then, the color information was instantly transformed into a statistic peak wavelength distribution in less than 1 min by a self-developed statistical program (nanoparticleAnalysis). In addition, the interaction between ConA and dextran was proved with biospecific recognition. This approach is high-throughput and real-time, and is a convenient method to analyze carbohydrate-protein interaction at the single nanoparticle level efficiently.

KEYWORDS:

carbohydrate−protein interaction; dark field microscopy; single plasmonic nanoparticle

PMID:
25985863
DOI:
10.1021/acsami.5b02744
[Indexed for MEDLINE]

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