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Nat Methods. 2015 Jul;12(7):645-8. doi: 10.1038/nmeth.3405. Epub 2015 May 18.

In vivo single-cell labeling by confined primed conversion.

Author information

1
Department of Biosystems Science and Engineering (D-BSSE), ETH Zurich, Basel, Switzerland.
2
Department of Molecular and Computational Biology, University of Southern California, Los Angeles, California, USA.

Abstract

Spatially confined green-to-red photoconversion of fluorescent proteins with high-power, pulsed laser illumination is negligible, thus precluding optical selection of single cells in vivo. We report primed conversion, in which low-power, dual-wavelength, continuous-wave illumination results in pronounced photoconversion. With a straightforward addition to a conventional confocal microscope, we show confined primed conversion in living zebrafish and reveal the complex anatomy of individual neurons packed between neighboring cells.

PMID:
25984699
DOI:
10.1038/nmeth.3405
[Indexed for MEDLINE]

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