Format

Send to

Choose Destination
Methods Mol Biol. 2015;1311:335-48. doi: 10.1007/978-1-4939-2687-9_22.

Precise Genome Editing of Drosophila with CRISPR RNA-Guided Cas9.

Author information

1
Genetics Training Program, University of Wisconsin-Madison, Madison, WI, 53706, USA.

Abstract

The readily programmable CRISPR-Cas9 system is transforming genome engineering. We and others have adapted the S. pyogenes CRISPR-Cas9 system to precisely engineer the Drosophila genome and demonstrated that these modifications are efficiently transmitted through the germline. Here we provide a detailed protocol for engineering small indels, defined deletions, and targeted insertion of exogenous DNA sequences within one month using a rapid DNA injection-based approach.

PMID:
25981484
PMCID:
PMC4467458
DOI:
10.1007/978-1-4939-2687-9_22
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center