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Methods Mol Biol. 2015;1311:307-16. doi: 10.1007/978-1-4939-2687-9_20.

Creation and Analysis of a Virome: Using CRISPR Spacers.

Author information

1
Department of Plant Biology, Carnegie Institution for Science, Stanford University, 260 Panama Street, Stanford, CA, 94305, USA.

Abstract

Advances in sequencing technology have allowed for the study of complex and previously unexplored microbial and viral populations; however, linking host-phage partners using in silico techniques has been challenging. Here, we describe the flow-through for creation of a virome, and its subsequent analysis with the viral assembly and analysis module "Viritas," which we have recently developed. This module allows for binning of contigs based on tetranucleotide frequencies, putative phage-host partner identification by CRISPR spacer matching, and identification of ORFs.

PMID:
25981482
DOI:
10.1007/978-1-4939-2687-9_20
[Indexed for MEDLINE]

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