Format

Send to

Choose Destination
PLoS One. 2015 May 15;10(5):e0126259. doi: 10.1371/journal.pone.0126259. eCollection 2015.

Cardiac development in zebrafish and human embryonic stem cells is inhibited by exposure to tobacco cigarettes and e-cigarettes.

Author information

1
Department of Pathology, University of Washington School of Medicine, Seattle, Washington, United States of America; Center for Cardiovascular Biology, University of Washington School of Medicine, Seattle, Washington, United States of America; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, Washington, United States of America.
2
Department of Pathology, University of Washington School of Medicine, Seattle, Washington, United States of America; Department of Bioengineering, University of Washington School of Medicine, Seattle, Washington, United States of America; Department of Medicine/Cardiology, University of Washington School of Medicine, Seattle, Washington, United States of America; Center for Cardiovascular Biology, University of Washington School of Medicine, Seattle, Washington, United States of America; Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, Washington, United States of America.

Abstract

BACKGROUND:

Maternal smoking is a risk factor for low birth weight and other adverse developmental outcomes.

OBJECTIVE:

We sought to determine the impact of standard tobacco cigarettes and e-cigarettes on heart development in vitro and in vivo.

METHODS:

Zebrafish (Danio rerio) were used to assess developmental effects in vivo and cardiac differentiation of human embryonic stem cells (hESCs) was used as a model for in vitro cardiac development.

RESULTS:

In zebrafish, exposure to both types of cigarettes results in broad, dose-dependent developmental defects coupled with severe heart malformation, pericardial edema and reduced heart function. Tobacco cigarettes are more toxic than e-cigarettes at comparable nicotine concentrations. During cardiac differentiation of hESCs, tobacco smoke exposure results in a delayed transition through mesoderm. Both types of cigarettes decrease expression of cardiac transcription factors in cardiac progenitor cells, suggesting a persistent delay in differentiation. In definitive human cardiomyocytes, both e-cigarette- and tobacco cigarette-treated samples showed reduced expression of sarcomeric genes such as MLC2v and MYL6. Furthermore, tobacco cigarette-treated samples had delayed onset of beating and showed low levels and aberrant localization of N-cadherin, reduced myofilament content with significantly reduced sarcomere length, and increased expression of the immature cardiac marker smooth muscle alpha-actin.

CONCLUSION:

These data indicate a negative effect of both tobacco cigarettes and e-cigarettes on heart development in vitro and in vivo. Tobacco cigarettes are more toxic than E-cigarettes and exhibit a broader spectrum of cardiac developmental defects.

PMID:
25978043
PMCID:
PMC4433280
DOI:
10.1371/journal.pone.0126259
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center